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Summer 2015
Mar 28, 2024
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BCHM 62000 - Protein Mass Spectrometry And Proteomics
Credit Hours: 2.00. The goals of this course are to introduce students to 1) basic principles of mass spectrometry, 2) the most common instruments used for protein mass spectrometry including the advantages and disadvantages of each, 3) the most common applications of protein mass spectrometry in biological research, 4) current approaches to quantitative protein mass spectrometry and their use in proteomic studies 5) the skills necessary to analyze mass spec data from a variety of experiment types including the ability to use and understand common database search programs, and 6) contemporary issues associated with large-scale proteomics experiments (including technical challenges and limitations) culminating in the ability to design appropriate experiments to answer a specific proteomic question. Prerequisite: Students need to have taken an undergraduate level biochemistry course and be familiar with basic aspects of protein structure that are taught in such courses. In the absence of an undergraduate biochemistry course, students should discuss their qualifications with the instructor before registering. Typically offered Spring.
2.000 Credit hours

Syllabus Available
Levels: Graduate, Professional, Undergraduate
Schedule Types: Lecture

Offered By: College of Agriculture
Department: Biochemistry


May be offered at any of the following campuses:     
      West Lafayette

Learning Outcomes: 1. Learn basic theoretical principles of mass spectrometry, with a focus on protein analysis. 2. Be knowledgeable in practical skills associated with mass spectral analysis of biological molecules, including interpretation, with a heavy emphasis on proteins. 3. Understand the basics of experimental design for the mass spectral analysis of proteins and other biological molecules. 4. Acquire an appreciation for current instrumentation and methods commonly used in protein mass spectrometry and the advantages and disadvantages of each. 5. Be able to read and critically evaluate primary literature pertaining to proteomic studies.

Restrictions:
Must be enrolled in one of the following Levels:     
      Graduate

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